In Vitro Studies on Different Parts of Trigonella cylindracea Desv.: Antimicrobial and Antibiofilm Avtivities
Keywords:Antibiofilm, Antimicrobial, Fenugreek, Ethanol extract, Trigonella
The aim of this study is to investigate, the antimicrobial and antibiofilm activities of ethanol extract prepared from the seeds, aerial parts and seed coats of Trigonella cylindracea Desv. Antimicrobial activity was determined by using standard microdilution method by five reference bacteria and three reference fungal strains. In addition, the effects of P. aeruginosa on biofilm formation and preformed biofilm were investigated by crystal violet staining method. Although the antimicrobial activity of ethanolic extracts on the yeasts included in the study (125-62.5 µg/mL) was slightly better than on bacteria (250-125 µg/mL), it was found that they generally showed moderate and low antimicrobial activity. Biofilm tests have shown that seed coat extract can reduce biofilm formation by 50% at sub-MIC (0.5X). It was also determined that the seed and seed coat extracts were able to reduce the preformed biofilm by 50% above the MIC (2X). In conclusion, our findings reveal that ethanolic extracts obtained from different parts of T. cylindracea have antibiofilm potential although their antimicrobial potentials are low/moderate. The literature review showed that the antimicrobial and antibiofilm potential of T. cylindracea was investigated for the first time in this study. Therefore, our findings provide important preliminary data to the literature on the antimicrobial and antibiofilm activity of this species.
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